Characterization of a Glycoside Hydrolase Family 157 Endo--1,3-Glucanase That Displays Antifungal Activity against Phytopathogens

Journal of agricultural and food chemistry(2023)

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beta-1,3-Glucan-degrading enzymes are widely used in fields such as food processing, plant protection, and breweries. In this work, we identified a glycoside hydrolase (GH) family 157 endo-beta-1,3-glucanase (BsGlc157A) from Bacteroides sp. M27 and characterized its biochemical properties, structural model, and antifungal activity. Enzymological characterization indicated that BsGlc157A performs its optimal catalytic activity at pH 6.0 and 40 degrees C. BsGlc157A adopted the classic (beta/alpha)(8) TIM-barrel structure. Two catalytic residues, the nucleophile (Glu215) and the proton donor (Glu123), were confirmed via structural modeling and site-directed mutagenesis. Moreover, BsGlc157A hydrolyzed curdlan into a series of oligosaccharides with polymerization degrees 2-5 and exhibited inhibitory effects on the hyphal growth of typical fruit pathogenic fungi (Monilinia fructicola, Alternaria alternata, and Colletotrichum gloeosporioides), thereby illustrating effective biocontrol activity. These results revealed the catalytic properties and the application potential of GH family 157 beta-1,3-glucanase, thus providing valuable biochemistry information about the group of carbohydrate-active enzymes.
beta-1,3-glucanase,glycoside hydrolase,antifungal activity,biochemical characterization,oligosaccharide
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