Neuroprotective effect of chrysophanol in Alzheimer disease via modulating the Ca²⁺/EGFR-PLC pathway

Chrysophanol (CHR) is an anthraquinone compound found in rhubarb, and it possesses neuroprotective properties. The purpose of this study was to gain a better understanding of its role in Alzheimer's disease (AD). In vivo study, D-galactose combined with intracerebral injection of beta-protein 25-35(A beta 25-35) were used to establish AD model rats. In vitro study, A beta(25-35) was used to induce AD model cells. Our results indicated that CHR improves learning and memory in AD model rats and provides protection against neuronal damage in both AD model rats and cells. Additionally, we observed that CHR suppressed the protein expression of p-tau, EGFR, PLC gamma, IP3R, and CAM, as well as the mRNA levels of tau, EGFR, PLC gamma, IP3R, and CAM. Furthermore, we have confirmed that CHR inhibited the fluorescence expression of calcium ions (Ca2+). In conclusion, the CHR may exert neuroprotective effects in AD by reducing tau phosphorylation through the Ca2+/EGFR-PLC gamma pathway.