Diversity in the radiation-induced transcriptomic temporal response of mouse brain tissue regions
arxiv(2024)
摘要
A number of studies have indicated a potential association between prenatal
exposure to radiation and late mental disabilities. This is believed to be due
to long-term developmental changes and functional impairment of the central
nervous system following radiation exposure during gestation. This study
conducted a bioinformatics analysis on transcriptomic profiles from mouse brain
tissue prenatally exposed to increasing doses of X-radiation. Gene expression
levels were assessed in different brain regions (cortex, hippocampus,
cerebellum) and collected at different time points (at 1 and 6 months after
birth) for C57BL mice exposed at embryonic day E11 to varying doses of
radiation (0, 0.1 and 1 Gy). This study aimed to elucidate the differences in
response to radiation between different brain regions at different intervals
after birth (1 and 6 months). The data was visualised using a two-dimensional
Uniform Manifold Approximation and Projection (UMAP) projection, and the
influence of the factors was investigated using analysis of variance (ANOVA).
It was observed that gene expression was influenced by each factor (tissue,
time, and dose), although to varying degrees. The gene expression trend within
doses was compared for each tissue, as well as the significant pathways between
tissues at different time intervals. Furthermore, in addition to
radiation-responsive pathways, Cytoscape's functional and network analyses
revealed changes in various pathways related to cognition, which is consistent
with previously published data [1] [2] [3], indicating late behavioural changes
in animals prenatally exposed to radiation.
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