Supplementary Figures 1-8 from Modeling Myeloma Dissemination <i>In Vitro</i> with hMSC-interacting Subpopulations of INA-6 Cells and Their Aggregation/Detachment Dynamics

Supplementary Figure 1. Principle and quantification of the V-well adhesion assay of fluorescently labeled myeloma cells adapted by Weetall et al. 2001.

Supplementary Figure 2. Validation of image cytometric analysis of cell cycle in four INA-6 cultures.

Supplementary Figure 3. Cell cycle analysis of INA-6 pellets gained from V-Well Adhesion assay (Fig. 3).

Supplementary Figure 4. Representative (one of the four independent sample sets as seen in Supplementary Figure 3) curve fitting analysis of cell cycle profiles generated by Image Cytometry.

Supplementary Figure 5. Correlation of RNAseq with qPCR Left: Validation of RNAseq results (Fig. 4) with qPCR showing the log2(foldchange expression) of 18 genes.

Supplementary Figure 6. Functional enrichment analysis by Metascape using genes that are differentially expressed between MSC-interacting subpopulations.

Supplementary Figure 7. Expression levels of adhesion genes that are downregulated and associated with survival (p < 0.01). Bone Marrow Plasma Cell (BMPC), Monoclonal Gammopathy of Undetermined Significance (MGUS), Smoldering Multiple Myeloma (sMM), Multiple Myeloma (MM), Multiple Myeloma Relapse (MMR).

Supplementary Figure 8. Expression levels of adhesion genes that are not downregulated and associated with survival (p < 0.01).