Algae-Leached DOM Inhibits the Hg(II) Reduction and Uptake by Lettuce in Aquatic Environments Under Light Conditions

The significant role of aquatic phytoplankton in global primary productivity, accounting for approximately 50% on an annual basis, has been recognized as a crucial factor in the reduction of Hg(II). In this study, we compared the efficiency of Hg(II) photoreduction mediated by three types of algae leaching dissolved organic matter (DOM) and humic acid (DOM-HA). Especially, we investigated the potential effects of algae-leached DOM on the photoreduction of Hg(II) and its subsequent uptake by lettuce, which serves as an indicator of Hg bioavailability for aquatic plants. The results revealed that under light conditions, the conversion of Hg(II) to Hg(0) mediated by algae-leached DOM and DOM-HA was 6.4-39.9% higher compared to dark condition. Furthermore, the free radical quenching experiment demonstrated that the reduction of Hg(II) mediated by DOM-HA was higher than algae-leached DOM, mainly due to its ability to generate superoxide anion (O2•−). Moreover, the photoreduction efficiences of Hg(II) mediated by algae-leached DOM were 29-18% lower compared to DOM-HA. The FT-IR analysis revealed that the production of -SH from algae-leached DOM led to the formation of strong metal-complexes, which restricts the reduction process from Hg(II) to Hg(0). Finally, the hydroponics experiment demonstrated that algae-leached DOM inhibited the bioavailability of Hg(II) to plants more effectively than DOM-HA. Our research emphasizes the significant functional roles and potential mechanisms of algae in reducing Hg levels, thereby influencing the availability of Hg in aquatic ecosystems.