PVP-AuNP Impedes Glycation Mediated Hen Egg White Lysozyme Aggregation Under Physiological Condition

Protein glycation a non-enzymatic protein modification, alters the structure of biomolecule leading to several neurodegenerative and other disorders. As onset of disorders due to protein glycation is primarily driven by the development of advanced glycation end products (AGEs), therapeutic intervention against related disorders by inhibiting AGEs production is imperative. Nanoparticles have recently gained more prominence as therapeutic agents in biological field such as medicine, drug discovery and diagnosis. In present study, we extensively investigated the effect of chemically synthesized polyvinylpyrrolidone conjugated gold nanoparticles (PVP-AuNP) on D-ribose induced glycation of hen egg white lysozyme (HEWL) under physiological conditions employing several biophysical and biochemical methods like absorption and fluorescence spectroscopy, static and dynamic light scattering, circular dichroism, alongwith fluorescence, and Atomic Force Microscopy. Our finding shows that AGEs formation was inhibited by PVP-AuNP at 37 °C, pH 7.4 over the period of 20 days. Interaction of gold nanoparticles prevented glycation induced misfolding and aggregation of lysozyme by stabilizing its native structure, which was evident with static light scattering, ThT, Congo red and ANS fluorescence coupled with CD spectroscopy. Further, by estimating carbonyl content and thiol group, our study suggests that PVP-AuNP possesses antioxidant property thus prevent the HEWL against glycation driven oxidative damage. Present study therefore elucidates that PVP-AuNP a significant antiglycation agent can be used against wide range of disorders induced by AGEs.