FIGURE 5 from Overcoming Xenoantigen Immunity to Enable Cellular Tracking and Gene Regulation with Immune-competent “NoGlow” Mice

Tumor cells expressing GFP, rtTA, and Luciferase successfully engraft in NoGlow mice. A, Diagram of triple-transgenic (3 ×) E0771 cells. GFP and rtTA are constitutively expressed and Luc is induced with the addition of doxycycline. B, 3 × E0771 (10⁶) were implanted into the mammary fat pad (MFP) of wild-type (WT) C57Bl/6 (n = 7), CAG-driven Full-body WT GFP/Luc expressing mice (n = 7), CMV cre littermates positive (n = 6) or negative (n = 7) for the NoGlow construct, or SCID beige (n = 4) mice. C, Overall (top) and individual (bottom) tumor growth in each group. No evidence of tumors was observed in Full-body GFP-Luc, NoGlow−, or C57Bl/6 animals by the end of experiment. D, Representative bioluminescence imaging of animals pre (left) or post (right) doxycycline diet introduction. Bottom, Luc was induced in SCID mice but to a lesser degree compared with NoGlow+ mice. E, Representative GFP imaging upon euthanasia of Noglow+, SCID, and Full-body GFP-Luc mice. Left, concentrated GFP signal in tumors of NoGlow+ and SCID compared with Full-body GFP-Luc mice. Right, visceral GFP signal only in Full-body GFP-Luc mice. F, Representative lungs of NoGlow+, NoGlow−, Full-body GFP-Luc, or SCID mice upon euthanasia. Lung metastasis can be visualized in NoGlow+ and SCID animals.