Nondestructive and cost-effective silkworm, Bombyx mori (Lepidoptera: Bombycidae) cocoon sex classification using machine learning

Sericulture is the process of cultivating silkworm cocoons for the production of silks. The quality silk production requires quality seed production which in turn requires accurate classification of male and female pupa in grainage centers. The challenges in the current methods of silkworm cocoon sex classification using manual observation lie in the time-consuming nature of the process, potential human error, and difficulties in accurately discerning subtle morphological differences between male and female cocoons. FC1 and FC2 single hybrid variety breed pupa are commonly used in south India for the production of high yielding double hybrid bivoltine silkworm seeds. In this study, 1579 FC1 and 1669 FC2 variety samples were used for the classification process. To overcome the challenges of present physical observation by expert employees, camera images of FC1 and FC2 cocoons were used in this study for sex classification. The proposed model used Histogram Oriented Gradient (HOG) feature descriptor of cocoon samples. Linear Discriminant Analysis (LDA) was applied on the feature vector to reduce the dimension and this feature matrix was given to the classical machine learning algorithms support vector machine (SVM), k-nearest neighbors (kNN), and gaussian naïve bayes for classification with stratified 10-fold cross validation. The results showed that for FC1 data HOG + LDA + Naïve Bayes performed better with a mean accuracy of 95.3