Efficient Biosynthetic Fabrication of Spidroins with High Spinning Performance

The unique 3D structure of spider silk protein (spidroin) determines the excellent mechanical properties of spidroin fiber, but the difficulty of heterologous expression and poor spinning performance of recombinant spider silk protein limit its application. A high-yield low-molecular-weight biomimetic spidroin (Amy-6rep) is obtained by sequence modification, and its excellent spinning performance is verified by electrospinning it for use as a nanogenerator. Amy-6rep increases the highly fibrogenic microcrystalline region in the core repeat region of natural spidroin with limited sequence length and replaces the polyalanine sequence with an amyloid polypeptide through structural similarity. Due to sequence modification, the expression of Amy-6rep increased by approximate to 200%, and the self-assembly performance of Amy-6rep significantly increased. After electrospinning with Amy-6rep, the nanofibers exhibit good tribopower generation capacity. In this paper, a biomimetic spidroin sequence design with high yield and good spinning performance is reported, and a strategy for electrospinning to produce an artificial nanogenerator is explored. A high yield and low molecular weight bionic spider silk protein (Amy-6rep)is obtained by sequence modification and verifies its excellent spinning performance by electrospinning, which can be used as nano generator. The expression of Amy-6rep is increased by about 200%, the self-assembly performance is significantly improved, and the nanofibers showed good tribopower generation ability after electrospinning. image