Green synthesis of bimetallic Se@TiO2NPs and their formulation into biopolymers and their utilization as antimicrobial, anti-diabetic, antioxidant, and healing agent in vitro

A promising combination of two nanoparticles that typically exhibit synergetic behaviors is called bimetallic nanoparticles. The synthesis of bimetallic selenium and titanium oxide nanoparticles (Se@TiO2NPs) in the current work was carried out using green creator Talaromyces pinophilus. A green technique was employed to create formulated nanoparticles into biopolymers (nanocomposite) using carboxymethylcellulose and starch. Se@TiO2NPs and nanocomposite characteristics were carried out via physicochemical including FITR and XRD and topographical characterization including FESEM and HRTEM. Se@TiO2NPs diameters were 25 nm and the nanocomposite was noticed as a nano matrix and was found to have confirmed nanostructures. More inhibitory action was observed using nanocomposite than Se@TiO2NPs against Staphylococcus aureus, Salmonella typhi, Enterococcus faecalis, Candida albicans, Aspergillus flavus, and Mucor circinelloid with inhibition zones of 21, 23, 25, 29, 16, and 18 mm using nanocomposite, while it was 19, 21, 24, 26, 13, and 12 mm using Se@TiO2NPs, respectively. Nanocomposite revealed low values of MIC (62.5, 31.25, and 7.8 µg/mL) in contrast to the MIC values of Se@TiO2NPs (125, 62.5, and 15.62 µg/mL) toward S. aureus, S. typhi, and C. albicans, respectively. DPPH scavenging