High Thermosensitivity of MDA-MB-231 Cells as a Prerequisite for Thermoradiosensitization of Triple-Negative Breast Cancer in Clinical Practice

This work is dedicated to analysis of the thermal sensitivity and efficiency of thermosensitization to γ-radiation of three cell lines of breast cancer of different molecular subtypes. It has been established that the cells of the studied lines differ in sensitivity to hyperthermia and, as a result, in the effectiveness of thermoradiosensitization, which raises the question of finding criteria for assessing the rationale of using hyperthermia in each specific case. It has been shown that the level of heat-responsive expression of the HSP70 gene and also the level of cell genomic instability can serve as such criteria. It was found that the efficiency of thermoradiosensitization is significantly higher in MDA-MB-231 cells compared to the cells of other lines studied. The data obtained suggest that the use of hyperthermia as a radiotherapy-sensitizing cotreatment may be particularly promising toward triple negative breast cancer.