Lipid Monolayer on Cell Surface Protein Templates Functional Extracellular Lipid Assembly

When the ancestors of men moved from aquatic habitats to the drylands, their evolutionary strategy to restrict water loss is to seal the skin surface with lipids. It is unknown how these rigid ceramide-dominated lipids with densely packed chains squeeze through narrow extracellular spaces and how they assemble into their complex multilamellar architecture. Here it is shown that the human corneocyte lipid envelope, a monolayer of ultralong covalently bound lipids on the cell surface protein, templates the functional barrier assembly by partly fluidizing and rearranging the free extracellular lipids in its vicinity during the sculpting of a functional skin lipid barrier. The lipid envelope also maintains the fluidity of the extracellular lipids during mechanical stress. This local lipid fluidization does not compromise the permeability barrier. The results provide new testable hypotheses about epidermal homeostasis and the pathophysiology underlying diseases with impaired lipid binding to corneocytes, such as congenital ichthyosis. In a broader sense, this lipoprotein-mediated fluidization of rigid (sphingo)lipid patches may also be relevant to lipid rafts and cellular signaling events and inspire new functional materials. The barriers of organisms to water loss are provided by extracellular lipids, but the mechanisms that sculpt these rather rigid lipid layers are not fully understood. In human skin, covalently bound ultra-long ceramides on the surface of corneocytes, the corneocyte lipid envelope, fluidize and rearrange neighboring extracellular matrix lipids, ensuring their malleability into a functional protective barrier against the environment.image