Interferometric reflectance imaging sensor for diagnosis and therapy

This chapter introduces the working principles and applications of interferometric reflectance imaging sensors (IRISs), which proved its success as a highly sensitive detection tool in diagnosis. Briefly, the surface chemistry of low-cost Si–SiO2 IRIS chips, probe molecules placement on the chip surface with microarray technology, data collection after molecular interactions, image analysis, and data processing steps are described in this section. Moreover, IRIS, which offers label-free and multiplex assay opportunities, has two different models depending on the camera features. Measurement of bioaccumulation depending on the binding of analyte on the chip surface is realized by low magnification IRIS model. On the other hand, with a high-magnification version of IRIS model, a single particle with nanosized can be detected and also visualized individually. The basic principles and successful applications of both the low magnification IRIS model based on the measurement of biomass accumulation and single-particle IRIS are also summarized in this chapter.