Bioactivity a mixture of spiked pepper fruit extract and fish-poison bean leaf extract against Spodoptera frugiperda (Lepidoptera: Noctuidae)

Abstract The active compounds on spiked pepper ( Piper aduncum ) fruit extract and fish-poison bean ( Tephrosia vogelii ) leaf extract have a potency used as botanical insecticides as alternative pest control. This study was conducted to determine the activity of mixed extracts of Spiked pepper fruit and Fish-poison bean leaf extract against the new invasive pest Spodoptera frugiperda larvae. This study was held from February 2021 to November 2021 at the Insect Bioecology Laboratory, Faculty of Agriculture, Andalas University, Padang. The experiment consists of a preliminary test and an advanced test. The advanced test was conducted using six concentrations and five replications of a mixture extract of Spiked pepper and Fish-poison bean. The parameters observed were larvae mortality, feeding inhibition activity (antifeedant), and larval development time. The result showed that the combination indexes of the mixed extract of Spiked pepper fruit and Fish-poison bean leaves (1:1) were additive with the value of LC 50 and LC 95 is 0,29% and 0,59%, respectively. Mixed extract of Spiked pepper fruit and Fish-poison bean leaves (1:1) caused larvae mortality, inhibited feeding activity, and inhibited S. frugiperda larvae growth. The formulation is needed to increase the mixed extract of Spiked pepper fruit and Fish-poison bean leaves (1:1) activity against S. frugiperda .