Insights into the growth parameters, mineral composition, protein and UHPLC–QTOF–LC/MS‐based non‐targeted metabolomic fingerprints of wheatgrass as a function of photoperiod and temperature

Summary Wheatgrass juice powder (WJP) obtained from four Indian wheat varieties grown under different photoperiod and temperature conditions were examined for growth parameters, chlorophyll content, mineral composition, protein and metabolomic profile. The percentage yield and total chlorophyll content of WJP varied from 2.5% to 9.12% and 1.08 to 4.76 mg g −1 , respectively. The WJP obtained from PBW‐752 showed the highest yield and total chlorophyll content. The mineral composition (Na, K, Ca and Mg) of WJP also showed variation with different photoperiod and temperature conditions. The electrophoretic pattern of total soluble proteins of WJP showed variability in the accumulation of high and low molecular weight polypeptides against different photoperiod and temperature conditions. The comprehensive metabolites profile of WJP was analysed by UHPLC–QTOF–liquid chromatography/mass spectrometry. Based on the retention times and m / z values, 1012 feature peaks in positive ionisation mode were detected. The metabolomic profile of WJP revealed 180 different classes of primary and secondary metabolites.