Methods to Determine Biogenic Amines in Wine by RP-HPLC

Biogenic amines can naturally be present in grapes or appear during the winemaking and/or aging processes, mainly due to the activity of microorganisms, such as lactic acid bacteria. Determining biogenic amines in wines is primarily performed by liquid chromatography with reversed-phase (RP) separation by C18 columns, using derivatisation reagents to promote its separation and detection. Nowadays, developing faster and inexpensive techniques or methodologies to apply in the wine industry is still challenging. Thus, the most used HPLC derivatisation methods to determine biogenic amines are presented, but also a simple dispersive solid-phase extraction clean-up/concentration method for selective and sensitive quantitation of biogenic amines in wines using benzoyl chloride derivatisation.