Localized phosphorylation of RNA Polymerase II by G1 cyclin-Cdk promotes cell cycle entry
bioRxiv (Cold Spring Harbor Laboratory)(2021)
摘要
Abstract The cell cycle is thought to be initiated by cyclin-dependent kinases (Cdk) inactivating transcriptional inhibitors of cell cycle gene-expression (1, 2) . In budding yeast, the G1 cyclin Cln3-Cdk1 complex is thought to directly phosphorylate Whi5, thereby releasing the transcription factor SBF and committing cells to division (3-7) . Here, we report that Cln3-Cdk1 does not phosphorylate Whi5, but instead phosphorylates the RNA Polymerase II subunit Rpb1’s C - t erminal d omain (CTD) on S 5 of its heptapeptide repeats. Cln3-Cdk1 binds SBF-regulated promoters (8) and Cln3’s function can be performed by the canonical S 5 kinase (9) Ccl1-Kin28 when synthetically recruited to SBF. Thus, Cln3-Cdk1 triggers cell division by phosphorylating Rpb1 at SBF-regulated promoters to activate transcription. Our findings blur the distinction between cell cycle and transcriptional Cdks to highlight the ancient relationship between these processes.
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关键词
rna polymerase ii,cell cycle,phosphorylation,cyclin-cdk
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