The core genome M5C plays an important role in methylation modification and immune infiltration of acute myelocytic leukemia samples

Abstract Currently, the pathogenesis of acute myelocytic leukemia(AML) is still unclear. We found the core genome M5C plays a vital role in methylation modification and immune infiltration of AML. At the same time, we created a new M5C score model to define the high and low-risk groups of AML.Our research showed the expression levels of the three molecular subtypes of M5C (C1, C2 and C3);as well as different clinical features ,the results showed significant differences in age\RUNX1-RUNX1T1 fusion, but not in RUNX1 mutation group.We constructed a prognostic risk model based on m5C phenotype from 417 samples in the GSE37642 data set and found 5 differential genes using lasso regression method. And the prognostic KM curve of the 5-gene signature was obtained, from which it can be seen that: all the five genes could significantly reduce the high and low risk of GSE37642 training set samples ( P < 0.05).Finally, the robustness of M5C related 5-gene signature for AML prediction was verified by internal and external data using single factor and multifactor COX regression analysis.5-gene signature has strong robustness and can play a stable prediction performance in external validation data sets (GSE12417, TCGA-LAML).