-Glucosidase on clay minerals: Structure and function in the synthesis of octyl glucoside

beta-Glucosidase is a biological macromolecule that catalyzes the hydrolysis of various glycosides and oligosac-charides. It may also be used to catalyze the synthesis of glycosides under suitable conditions. Carrier-bound 13-glucosidase can enhance the enzymatic activity in the synthesis of glycosides in organic solvent solutions, although the molecular mechanism regulating activity is yet unknown. This study investigated the impact of utilizing montmorillonite (Mmt), attapulgite (Attp), and kaolinite (Kao) as carriers on the activity of 13-glucosi-dase from Prunus dulcis (PdBg). When Attp was used as carriers, the molecular dynamic (MD) simulations found the distance between pNPG and the active site residues E183 and E387 was minimally impacted by the ad-sorptions, hence PdBg maintained about 81.3 +/- 0.89 % of its native activity. Out of the three clay minerals, the relative activity of PdBg loaded on Mmt was the lowest because of the highest electrostatic energy. The substrate channel of PdBg on Kao is directed towards the surface, limiting the accessibility of substrates. Secondary structure and conformation studies revealed that the conformational stability of PdBg in solvent solutions was enhanced by coupling to Attp. Unlike dimethyl sulfoxide (DMSO), N,N-dimethylformamide (DMF) and 1,2-dime-thoxyethane (DME), tert-butanol (t-BA) did not penetrate into the active site of PdBg interfering with its binding to the substrate. The maximum yield of n-octyl-13-glucoside (OGP) synthesis catalyzed by Attp-immobilized PdBg reached 48.3 %.