Differential Runx3, Eomes, and T-bet expression subdivides MS-associated CD4⁺ T cells with brain-homing capacity

Multiple sclerosis (MS) is a common and devastating chronic inflammatory disease of the CNS. CD4(+) T cells are assumed to be the first to cross the blood-central nervous system (CNS) barrier and trigger local inflammation. Here, we explored how pathogenicity-associated effector programs define CD4(+) T cell subsets with brain-homing ability in MS. Runx3- and Eomes-, but not T-bet-expressing CD4(+) memory cells were diminished in the blood of MS patients. This decline reversed following natalizumab treatment and was supported by a Runx3(+)Eomes(+)T-bet- enrichment in cerebrospinal fluid samples of treatment-naive MS patients. This transcription factor profile was associated with high granzyme K (GZMK) and CCR5 levels and was most prominent in Th17.1 cells (CCR6(+)CXCR3(+)CCR4(-/dim)). Previously published CD28- CD4 T cells were characterized by a Runx3(+)Eomes(-)T-bet(+) phenotype that coincided with intermediate CCR5 and a higher granzyme B (GZMB) and perforin expression, indicating the presence of two separate subsets. Under steady-state conditions, granzyme K-high Th17.1 cells spontaneously passed the blood-brain barrier in vitro. This was only found for other subsets including CD28(-) cells when using inflamed barriers. Altogether, CD4(+ )T cells contain small fractions with separate pathogenic features, of which Th17.1 seems to breach the blood-brain barrier as a possible early event in MS.