Wild ruminants` intestinal microflora identification by the maldi-tof-mass spectrometry proteomic technique

Wild ruminants, such as Carpathian fallow deer (Dama dama) and Carpathian chamois (Rupicapra rupicapra carpatica), represent an important part of the natural ecosystem of Romania. These animals had a significant impact on biodiversity and the well-being of the ecosystem through their pasture behaviour and interaction with the surroundings. Because of their ecological importance, wild ruminants are hosts for microorganisms and bacteria. The subject of the study was to research and compare the bacterial profiles of wild ruminants, including Carpathian fallow deer and Carpathian chamois. This study used common identification and isolation methods, as well as modern identification techniques like proteomic technique, and MALDI-TOF Ms technique, for high-accuracy information regarding the bacterial flora of the digestive system. This paper aimed to study and observe newly developed antibiotic resistance profiles in bacterial flora isolated from these wild species and to compare our data with the existing ones in the literature. Our goal was to understand the relationship between these wild animals and their intestinal microbiota, and to know the potential microbial risks associated with their late-developed antibioresistency phenomenon. The low resistance to the tested antimicrobials denotes that the wild animals (Carpathian fallow deer and Carpathian chamois) from which the bacterial strains originated have not yet been subjected to high infectious pressures, especially those obtained from fallow deer (Dama dama).