SERS detection of apramycin and kanamycin through sliver nanoparticles modified with -cyclodextrin and -iron oxide

Using sliver nanoparticles modified with beta-cyclodextrin and alpha-iron oxide (beta-CD/alpha-Fe2O3@AgNPs) as surface-enhanced Raman spectroscopy (SERS) substrate, two sensitive methods for detecting apramycin and kanamycin were established. The synthesized beta-CD/alpha-Fe2O3@AgNPs were characterized through ultraviolet visible (UV-vis) spectroscopy, transmission electron microscope (TEM), X-ray diffraction (XRD) and thermogravimetric analyses (TGA). The interactions of the two drugs and substrate were researched by UV-vis absorption and fourier transform infrared (FT-IR). The linear relationship between apramycin/kanamycin and SERS intensity was observed. The limits of detection (LODs) (S/N = 3) were 3.42 and 0.31 nmol/L. The two SERS methods were effectively applied to detect apramycin and kanamycin in beef samples and commercial injection. The recoveries were 96.84 - 102.20% with relative standard deviations (RSD) of 0.6-4.0% for apramycin and 95.67 - 103.18% with RSD of 1.4 - 2.5% for kanamycin, respectively.