Highly efficient -lactamase assay applying poly-dimethylacrylamide-based surface functionalization with -lactam antibiotics and -lactamase inhibitors

In recent decades, the rise of beta-lactamases has substantially led to the emergence and wide spread of antibiotic resistance posing a serious global health threat. There is growing need for the development of rapid, cost-effective and user-friendly diagnostic assays for the accurate detection of beta-lactamases to optimize patient outcomes and prevent the spread of multidrug-resistances. In this article, we present a poly-dimethylacrylamide (PDMA)-based surface functionalization to immobilize beta-lactam antibiotics and beta-lactamase inhibitors of different subclasses. Immobilization was induced via UV-crosslinking through C,H-insertion reactions. The functional coatings were successfully applied in a highly efficient assay for the determination of recombinant beta-lactamases as well as beta-lactamases isolated from clinically relevant bacterial strains. Thus, this method describes an innovative approach with several significant benefits for diagnostic applications: the creation of specific detection platforms tailored for beta-lactamase activity, the development of high-throughput diagnostic assays and benefits regarding stability and shelf-life. Furthermore, this method is highly adaptable to other surfaces, antibiotics, and analytes, offering far-reaching implications for various biomedical, environmental, and antimicrobial applications. Immobilization of antibiotics to surfaces via PDMA by UV-crosslinking providing a highly efficient diagnostic beta-lactamase assay. The method is transferable to different substrate materials and devices as well as antibiotics and chemical substances.