Biological effects of air pollution on the function of human skin equivalents

The World Health Organization reports that 99% of the global population are exposed to pollution levels higher than the recommended air quality guidelines. Pollution-induced changes in the skin have begun to surface; however, the effects require further investigation so that effective protective strategies can be developed. This study aimed to investigate some of the aging-associated effects caused by ozone and particulate matter (PM) on human skin equivalents. Full-thickness skin equivalents were exposed to 0.01 mu g/mu L PM, 0.05 mu g/mu L PM, 0.3 ppm ozone, or a combination of 0.01 mu g/mu L PM and 0.3 ppm ozone, before skin equivalents and culture medium were harvested for histological/immunohistochemical staining, gene and protein expression analysis using qPCR, Western blotting, and ELISA. Markers include MMP-1, MMP-3, COL1A1, collagen-I, 4-HNE, HMGCR, and PGE2. PM was observed to induce a decrease in epidermal thickness and an enhanced matrix building phenotype, with increases in COL1A1 and an increase in collagen-I protein expression. By contrast, ozone induced an increase in epidermal thickness and was found to induce a matrix-degrading phenotype, with decreases in collagen-I gene/protein expression and increases in MMP-1 and MMP-3 gene/protein expression. Ozone was also found to induce changes in lipid homeostasis and inflammation induction. Some synergistic damage was also observed when combining ozone and 0.01 mu g/mu L PM. The results presented in this study identify distinct pollutant-induced effects and show how pollutants may act synergistically to augment damage; given individuals are rarely only exposed to one pollutant type, exposure to multiple pollutant types should be considered to develop effective protective interventions. Skin equivalents were exposed to particulate matter and/or ozone before being assessed for gene and protein markers associated with dermal integrity, inflammation, and the skin barrier. Ozone induced significant increases in epidermal thickness, MMP expression and inflammatory marker PGE2. Particulate matter alone induced no significant changes; however, when combined with ozone synergistic effects were observed. These results identify distinct pollutant-induced effects and demonstrate how pollutants may act synergistically to augment damage. This should be considered when developing effective protective interventions.image