Dissolved organic compounds in shale gas extraction flowback water as principal disturbance factors of soil nitrogen dynamics.

Flowback water, a by-product of shale gas extraction, represents an extremely complex industrial wastewater characterized by high organic compounds content and high salinity. The prospect of flowback water entering the soil through various approaches concerns regarding its ecological risk. Nitrogen mineralization (Nmin), a key rate-limiting step in the soil N cycle, might be adversely affected by flowback water. Nonetheless, no previous studies have examined the effects of flowback water on soil Nmin rates, let alone quantified the relative contributions of the major components of flowback water to changes in Nmin rates. Therefore, this study investigated the effects of flowback water and sterile flowback water at two different concentrations on the Nmin rates of three distinct soil types. This study aimed to elucidate the predominant influence of the key constituents within flowback water on the changes in soil Nmin rates. The results showed that soil soluble salt content, dissolved organic carbon (DOC) and dissolved nitrogen (DN) content significantly increased by 8.37 times, 9.5 % and 26.4 %, respectively, in soils contaminated by flowback water. In comparison with the control group, the introduction of flowback water resulted in a significant 25.9 % reduction in Nmin rate in sandy soils. Conversely, in clay and loam soils, there was a significant increase in Nmin rates by 44.9 % and 131.8 % respectively. Throughout the incubation period, leucine-aminopeptidase activity exhibited irregular fluctuations. Analysis of microbial communities demonstrated that flowback water only significant impacted soil rare microbial taxa, inducing a significant increase in alpha diversity for sandy, clay, and loamy soils by -16.9 %, 10.12 %, and 1.63 %, respectively. Linear regression and random forest analyses indicated that alterations in soil DOC:DN ratio and salt content were responsible for changes in soil Nmin rates within flowback water-contaminated soils. In contrast, only salt content significantly contributed to shifts in alpha diversity among soil rare microbial taxa. Structural equation modeling highlighted that the total effect of dissolved organic compounds (DOC and DN, λ = 0.64) from flowback water was greater than the total effect of salinity (λ = 0.24) on soil Nmin rates. In conclusion, our findings imply that dissolved organic compounds within flowback water play pivotal roles in determining soil Nmin rates. To the best of our knowledge, this is the first study to reveal the effects of major components in the flowback water on soil N mineralization rates.