Dual drug-loaded core-shell nanofibers membranes via emulsion electrospinning and their controllable sustained release property

With the improvement of the medical level and the need for pathological research, loading multiple drugs and controlling the burst release and differential release of multiple drugs has become a current research hotspot. In this work, a spherical VB12/Lys/CMC colloidal particle of approximately 400 nm was prepared from sodium carboxymethylcellulose (CMC), lysozyme (Lys) and hydrophilic drug (VB12) by macromolecular self-assembly technique. Subsequently, an oil-in-water emulsion was obtained by using VB12/Lys/CMC colloidal particles as emulsifiers, gelatin as spinning aid, and corn oil containing hydrophobic drug (VD3) as oil phase. Dual drug-loaded core-shell nanofibers were further obtained by emulsion electrospinning. The sustained release effect of drugs in nanofibers was investigated. The results indicate that nanofibers have good sustained release effects on VB12 and VD3 over a long period of time, and the sustained release effect is controllable. The kinetic data of sustained release indicate that the main mechanism of sustained release is Fick diffusion. In vitro cytotoxicity test showed that the prepared nanofibers had good biocompatibility. In this work, a new method for preparing dual-loaded core-shell nanofibers is presented, which has potential application in the field of combined therapy for complex diseases.