Sodium caseinate medium promotes the in vitro digestion of starch: An insight into the macromolecular crowding effect

The macromolecular crowding effects of polysaccharides can alter the activity of digestive enzymes; however, whether protein crowding affects the digestive properties of starch remains unknown. Herein, the interaction of sodium caseinate (NaCas) with starch and pig pancreas alpha-amylase (PPA) and their effects on enzyme activity and starch digestion were studied. NaCas delayed gelatinization and reduced the leaching amount of amylose and increased the relative content of easily digestible starch. The ratio of the ordered structure (alpha-helix and beta-sheet) to disordered structure (beta-turns) of PPA increased with NaCas concentration, indicating that NaCas maintained the conformational stability of the enzyme and thereby accelerated the rate of enzymatic hydrolysis. This study demonstrates the detailed mechanism of NaCas-induced enhancement of starch digestibility and suggests a nonnegligible macromolecular crowding effect should be considered when evaluating the function of food macromolecules.