Carboxyl groups on carbon nanodots as co-reactant sites for anodic electrochemiluminescence of tris(2,2-bipyridine)ruthenium(II)

Carbon nanodots (C-dots) with good biocompatibility have been extensively utilized as co-reactants for electrochemiluminescence (ECL) of the tris(2,2 '-bipyridine) ruthenium(II) (Ru(bpy)32+) system. However, the ECL intensity of this system is still relatively low and the mechanism of C-dots as co-reactants remains unclear, which greatly limits its further application in bio-analysis. In this work, we revealed that the carboxyl groups on C-dots are co-reactant sites for Ru(bpy)32+ ECL by systematically investigating the contribution of carboxyl, hydroxyl and carbonyl groups on the surface of C-dots to the ECL intensity. Further treatment with hydrogen peroxide to increase the carboxyl-group content on C-dots resulted in a 10-fold increase in ECL intensity over the original Ru(bpy)32+/C-dots system. This work provides new insights for the rational design of ECL systems with C-dots as co-reactants and offers new chances for further applications of C-dots in the field of ECL.