Reduced Energy Per Cycle, a Marker of Glutamatergic Synaptic Strength, in Individuals Diagnosed with PTSD and Depression

ABSTRACT Trauma and chronic stress are believed to induce and exacerbate psychopathology by disrupting glutamate synaptic strength. However, in vivo in human methods to estimate synaptic strength are limited. In this study, we established a novel putative biomarker of glutamatergic synaptic strength, termed energy-per-cycle (EPC). Then, we used EPC to investigate the role of prefrontal neurotransmission in trauma psychopathology. Healthy control (n=18) and patients with posttraumatic stress (PTSD; n=16) completed 13 C-acetate magnetic resonance spectroscopy scans to estimate prefrontal EPC, which is the ratio of neuronal energetic needs per glutamate neurotransmission cycle (V TCA /V Cycle ). Patients with PTSD were found to have 28% reduction in prefrontal EPC ( t =3.0; df =32, p =0.005). There was no effect of sex on EPC, but age was negatively associated with prefrontal EPC across groups ( r =–0.46, n=34, p =0.006). Controlling for age did not affect the study results. The feasibility and utility of EPC were established. Patients with PTSD were found to have reduced prefrontal glutamatergic synaptic strength. These findings suggest that reduced glutamatergic synaptic strength may contribute to the pathophysiology of PTSD and could be targeted by new treatments. Highlights Glutamatergic synaptic strength is critical for brain function in health and disease. In vivo in human methods to estimate glutamatergic synaptic strength are limited. We here propose a new approach to estimate glutamatergic synaptic strength. The new method employs carbon-13 magnetic resonance spectroscopy ( 13 C MRS). The utility of the new approach was demonstrated in posttraumatic stress disorder (PTSD).