The Effect of Dietary Synbiotics in Actively Racing Standardbred Horses Receiving Trimethoprim/Sulfadiazine

Simple Summary: Synbiotics are often provided to horses receiving antibiotics to protect against disturbances of gut microbioma, despite a lack of evidence for efficacy. The purpose of this study was to evaluate the effect of a synbiotic (PROBIOPlus (TM)) in horses receiving antibiotics. Sixteen actively racing Standardbred horses were randomly allocated to one of four groups: antibiotics (10 days; AB), synbiotic (28 days; PBP), PBP + AB, or Control. Make-up of the bacterial gut population was assessed, as well as indicators of manure quality. The PBP + AB group had a significantly different population of bacteria in their manure compared with all other groups. Most of the differences were found in bacterial populations that function to degrade fiber, including Fibrobacter and Ruminococcaceae. The Fibrobacter population was significantly higher in AB and PBP + AB horses than Control. For Ruminococcaceae, Control was significantly higher than AB and PBP during antibiotic treatment, and PBP + AB horses were significantly higher than PBP horses after antibiotic treatment. In conclusion, these data provide support for the ability of PROBIOPlus (TM) to maintain healthy gastrointestinal microbiome during antibiotic treatment. Synbiotics are often provided to horses receiving antibiotics to protect against microbiome disturbances, despite a lack of evidence for efficacy. The purpose of this study was to evaluate the effect of a synbiotic product in horses receiving antibiotics. Sixteen actively racing Standardbred horses were randomly allocated (four-way crossover) to one of four groups: antibiotics (10 days; AB), synbiotics (28 days; PROBIOPlusTM; PBP), PBP + AB, or Control. The fecal microbiome was investigated using 16S rRNA sequencing, and fecal dry matter (DM; %), pH, and scores (FS; 0-9) were measured. Data were analyzed with two-way ANOVA. Results found microbiota differences in community membership between PBP + AB and all other treatments during and after antibiotic treatment. During antibiotic treatment, AB and PBP + AB were significantly different from Control. After antibiotic treatment, PBP + AB was significantly different from all other treatments. The few differences found in relative abundance of phyla or predominant genera were mostly in fiber degrading bacteria. The Fibrobacter population was significantly higher in AB and PBP + AB horses than Control. Unclassified Ruminococcaceae was significantly higher in Control than AB and PBP. After antibiotic treatment, PBP + AB horses were significantly higher than PBP horses. In conclusion, these data provide support for the ability of PROBIOPlusT to maintain healthy gastrointestinal microbiome during antibiotic treatment.