Genetic Diversity of Ustilaginoidea virens in Heilongjiang Province

In recent years,the occurrence of rice false smut has become increasingly serious and the area of damage has expanded year by year,so the false smut has become one of the diseases which have seriously affected rice yield and quality in Heilongjiang Province.However,the genetic diversity research on false smut in Heilongjiang Province was relatively weak.In the current investigation,seven polymorphic SSR markers were taken to analyze the genetic diversity among 89 strains of the pathogens of rice false smut collected from five main rice growing areas in Heilongjiang Province,China.The results showed that the amplified bands of each pair of primers were between 2 and 7.A total of 43 bands were obtained,and each primer was expanded for 4.8 bands.The genetic similarity coefficient(GSC)between strains by using SSR molecular marker analysis showed that the GSC of the strain of Ustilaginoidea virens was 0.613-0.955,with an average of 0.741.According to the results cluster analysis,when the genetic distance was 0.73,89 strains were divided into three groups,and five strains of rice smut strains were distributed in three populations,and strains in Harbin City were mainly distributed in group Ⅲ.In Mudanjiang City,the strains were mainly distributed in groups Ⅰ and Ⅲ,and the strains in Suihua City were mainly distributed in groups Ⅰ and Ⅱ,and the strains in Jiamusi City were evenly distributed in groups Ⅰ,Ⅱ and Ⅲ,and the strains in Qiqihar City were mainly distributed in groups Ⅱ and Ⅲ.The results showed that the genetic background of Ustilaginoidea virens in Heilongjiang Province was relatively low in complexity,and the overall inheritance of the strain was relatively stable.