"Gearing" up for dynamin-catalyzed membrane fission

Endocytic dynamins self-assemble into helical scaffolds and utilize energy from GTP hydrolysis to constrict and sever tubular membranous necks of budded endocytic inter mediates. They bind the membrane using a pleckstrinhomology domain (PHD). The PHD is characterized by four unstructured loops, two of which partially insert into the membrane. Recent studies reveal that loop insertion lowers the bending rigidity of the membrane and that mutations in these two loops produce separable and opposite effects on the efficiency of dynamin-catalyzed membrane fission. Here, we review the current understanding of dynamin-catalyzed membrane fission and attempt to reconcile contrasting notions that have emerged from biochemical and cellular studies evaluating the role of the PHD in this process. We propose that two membrane-inser ting loops act as "gears" that define the catalytic efficiency of the dynamin helical scaffold in membrane fission.