Temozolomide-fatty acid conjugates for glioblastoma multiforme: In vitro and in vivo evaluation.

Glioblastoma multiforme (GBM) is the deadliest brain tumor with a poor prognosis and limited therapeutic options. Temozolomide (TMZ) is the first-line chemotherapeutic agent used for the treatment of GBM; however, it suffers from several limitations, including short half-life, rapid metabolism, <1% brain bioavailability, methyl guanine methyl transferase (MGMT) based chemoresistance, and hematological toxicities. Several approaches have been adopted to overcome these limitations, particularly using nanotechnology-based systems, but its physicochemical properties make it challenging to load into these nanocarriers. In the current research, we conjugated TMZ with different fatty acids, i.e., linoleic acid (LA), oleic acid (OA), and palmitic acid (PA), to obtain TMZ-fatty acid conjugates which are comparatively hydrophobic, less prone to degradation and potent. These conjugates were thoroughly characterized using H NMR spectroscopy, HR-MS, FTIR, and UV-visible spectroscopy. The synthesized conjugates TOA (6R), TLA (6R), and TPA (6R) showed an IC of 101.4, 67.97, and 672.04 μM, respectively in C cells and 428.257, 366.43 and 413.69 μM, respectively in U87-MG cells. On the other hand, the free TMZ showed an IC of 1098.3 μM and 564.23 μM in C and U87-MG, respectively. The in vivo efficacy of the TMZ-fatty acid conjugates was evaluated in the C-induced orthotropic rat glioblastoma model, wherein the TMZ-fatty acid conjugate showed improved survival rate (1.6 -fold) and overall health of the animals. Collectively, the conjugation of fatty acids with TMZ improves its anticancer potential against GBM.