[Cloning and expression profile of ZFP36L1 gene in goat].

The purpose of this study was to clone and characterize the (zinc finger protein 36-like 1) gene, clarify its expression characteristics, and elucidate its expression patterns in different tissues of goats. Samples of 15 tissues from Jianzhou big-eared goats, including heart, liver, spleen, lung and kidney were collected. Goat gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR), then the gene and protein sequence were analyzed by online tools. Quantitative real-time polymerase chain reaction (qPCR) was used to detect the expression level of in intramuscular preadipocytes in different tissues and adipocytes of goat at different differentiation stages. The results showed that the length of gene was 1 224 bp, and the coding sequence (CDS) region was 1 017 bp, encoding 338 amino acids, which was a non-secretory unstable protein mainly located in nucleus and cytoplasm. Tissue expression profile showed that gene was expressed in all selected tissues. In visceral tissues, the small intestine showed the highest expression level ( < 0.01). In muscle tissue, the highest expression level was presented in longissimus dorsi muscle ( < 0.01), whereas the expression level in subcutaneous adipose tissue was significantly higher than that in other tissues ( < 0.01). The results of induced differentiation showed that the expression of this gene was up-regulated during adipogenic differentiation of intramuscular precursor adipocytes ( < 0.01). These data may help to clarify the biological function of the gene in goat.