Superoxide Dismutase Due To Occupational Metals Exposure To Nickel On Mutant P53 Dental Technicians

Abstract Background: Nickel levels in dental technicians' blood serum are significantly linked to the generation of free radicals and catalyze the development of reactive oxygen species (ROS) (ROS). An increase in ROS causes oxidative stress and a p53 gene mutation. Superoxide dismutases (SODs) are important antioxidant enzymes that help the body fight oxidative stress. Purpose: The goal of this study is to see if dental technician occupational exposure to Ni contributes to the occurrence of p53 mutations and lower SOD activity in dental technicians. Methods: Blood samples were taken for 3 types of tests, namely Ni examination, with Atomic Absorbance Spectrophotometry (AAS), p53 mutant protein examination by ELISA method, and examination of SOD enzyme activity by Assay Method. Result: The concentration of nickel in dental technicians' blood samples is higher than in controls. Dental technician blood samples have lower levels of SOD than controls. Between dental technicians and controllers, there is a major difference. A negative connection exists between Ni and SOD, a positive correlation exists between Ni and p53mutant, and a negative correlation exists between SOD and p53mutant, according to the Spearman correlation test. Conclusion: A high nickel concentration in the blood serum of a dental worker raises the level of p53 mutants and lowers SOD activity, because p53 mutants have a negative dominant effect on wild p53 types, if wild-type p53 levels decline, p53 mutant levels may rise. Trial Registration: No: 149 / HRECC.FODM / VIII / 2017.