Comparison of Proliferation and Differentiation of Hanwoo Muscle Satellite Cells and C2C12 Myoblast Cells According to Culture Temperature

Abstract To improve culture efficiency of Hanwoo muscle satellite cells, these cells were cultured at different temperatures. Hanwoo muscle satellite cells were compared with C2C12 cells to observe proliferation and differentiation at culture temperatures of 37 ℃ and 39 ℃ and determine the possibility of using them as cultured meat. Immunofluorescence staining using Pax7 and Hoechst, both cells cultured at 37 ℃ proliferated better than cultured at 39 ℃ (p < 0.05). When differentiated cells were stained with myosin and Hoechst, there was no significant difference in root canal thickness and Fusion index. In Western blotting analysis, Hanwoo muscle satellite cells were no significant difference in the expression of myosin or cytochrome C between cells differentiated at the two temperatures. C2C12 cells were no significant difference in the expression of myosin between cells differentiated at the two temperatrues. In RT-qPCR analysis, Hanwoo muscle satellite cells cultured at 39 ℃ had significantly (p < 0.05) higher expression levels of MyHC, MRF4, and myoglobin than those cultured at 37 ℃. C2C12 cells cultured at 39 ℃ showed significantly (p < 0.05) higher expression levels of myogenin and myoglobin than those cultured at 37 ℃. To increase culture efficiency of Hanwoo muscle satellite cells, proliferating at 37 ℃ and differentiating at 39 ℃ are appropriate. Since results of temperature differences of Hanwoo muscle satellite cells were similar to those of C2C12 cells, they could be used as a reference for producing cultured meat using Hanwoo satellite cells.