Extraction and chemoprofiling study of Eulophia herbaceae Lind. tubers for colorectal cancer

Colon cancer is a great evildoer behind mortality around the world.Some non-developed regions like China and India stand in the third position in this regard.Maximum of the presently accessible colon cancer therapeutic choices are exorbitant and come up with hazards.However, traditionally available herbal derivatives or medications are comparatively innocuous.One prominent example is -sitosterol (BS), derived from Eulophia herbaceae Lind.(EH), exhibiting antineoplastic potential against malignancies associated with colon, lung, breast, etc.The ultimate goal of the present research was to isolate and explore the impact of BS from EH tubers.BS has been extracted and isolated from tubers' metabolic extract (MTE), further characterized by UV, IR, TLC, 1HNMR, GC-MS and 13C NMR techniques.Further, validation of pharmacological activity by employing sulforhodamine B (SRB) cytotoxicity assay on (COLO-205) confirmed its anticancer potential.The BS demonstrated > 80 g/ml of lethal concentration value (LC 50 ) as well as a total growth inhibition value (TGI).The Concentration of average growth inhibition (GI 50 ) for BS was less than 21.0 µg/ml against a cell line that has been found to possess significant anticancer potential against human colon cancer (COLO-205).From the present research, it can be concluded that the tubers of EH containing BS possess paramount anticancer potential against human colon cancer and can be considered for further development as biomedicine.