Supplementary Figures S1-S9 from CXADR-Mediated Formation of an AKT Inhibitory Signalosome at Tight Junctions Controls Epithelial–Mesenchymal Plasticity in Breast Cancer

This file includes supplementary figures S1-S9: Figure S1. Immunofluorescence and western blot results showing differences in CXADR expression between EpH4, EpRas and EpXT cells. Figure S2. Western blots showing how incubatino with an AKT inhibitor, or knockdown of CXADR affects EMT markers in EpRas cells. Figure S3. Immunofluorescence images showing co-localization of CXADR with PHLPP2, PTEN and MAGI-1 in EpH4 cells. Figure S4. Results from Co-IP experiments, protein stability assays, and confuency experiments. Figure S5. Immunofluorescence staining of PHLPP2 and PTEN in the intestinal epithelium of CXADR-deficient mice. Figure S6. Kaplan-Meier curves showing how the expression of CXADR and other junction proteins correlates with overall survival in the GOBO database. Figure S7. Kaplan-Meier curves showing how the expression of MAGI1, PTEN and PHLPP2 correlates with overall survival in the GOBO database. Figure S8. Schematic diagram showing the expression of CXADR in 51 human breast cancer cells. Immunofluorescence and western blot results showing how overexpression of CXADR affects EMT markers and signalosome components in breast and lung cancer cells. Figure S9. Scatter plots showing variability in staining of MAGI-1, PTEN, PHLPP2 and E-cadherin in luminal A and basal tumors.