Supplementary Figures 1 - 10, Tables 1 - 2 from CX3CL1 Promotes Breast Cancer via Transactivation of the EGF Pathway

PDF file - 1052K, S1. CX3CL1 staining in mammary glands of WT and WT and CX3CL1+ mice. S2. Staining of CX3CR1 in tumors and mammary glands of Tg-neu mice. S3. Effects of Ad-CX3CL1or Ad-LacZ injection in tumor proliferation, apoptosisand angiogenesis. S4. CX3CL1 overexpression increases breast carcinogenesis in Tg-neu mice. S5. CX3CL1 or CX3CR1 mRNA expression in breast cancer cell lines with distinct metastatic potential. S6. CX3CL1 expression in a panel of breast cancer cell lines. S7. CX3CL1 induces EMT in breast cancer cells. S8. Lack of lymphoid metastases in Ad-CX3CL1-injected Tg-neu mice. S9. CX3CL1 induces transactivation of the EGF pathway. S10. ErbB ligand expression in T47D cells. S11. CX3CL1 deficiency does not affect the structure and composition of the mammary gland in Tg-neu mice. S12. Hemizygous CX3CL1 mRNA expression in T-neu-CX3CL1+/-. Supplemental Table 1. Media used to culture the cancer cell lines. Supplemental Table 2. Primers and annealing conditions used for PCR.