Supplementary Figures S1-S10 from miR340 Suppresses the Stem-like Cell Function of Glioma-Initiating Cells by Targeting Tissue Plasminogen Activator

Supplementary Figures S1-10 Supplementary Figure S1. Characterization of hGICs demonstrates the stemness nature of the cells. Supplementary Figure S2. miR-340 transfection causes a markedly elevated level of miR-340 expression in hGICs compared with the control. Supplementary Figure S3. miR-340 overexpression in glioma cell lines moderately inhibits tumorigenesis in vivo. Supplementary Figure S4. miR-340 overexpression affects the expression of the genes related to two signaling pathways. Supplementary Figure S5. Inhibition of PLAT expression by miR-340 overexpression in hGICs and co-immunolocalization of PLAT and CD15 in GBM tissues. Supplementary Figure S6. Knockdown efficiency of the PLAT-shRNA expression vector. Supplementary Figure S7. Effects of PLAT-shRNA are due to the specific inhibition of PLAT in hGICs. Supplementary Figure S8. Inhibitory effects of miR-340-overexpressing hGICs on in vitro cell functions are recovered by PLAT introduction. Supplementary Figure S9. Microarray analysis on the expression of c-Met, CD44, Sox2, DNMT1, ANXA2, and CDKN1A genes in hGICs by miR-340 overexpression. Supplementary Figure S10. Survival curves of glioma patients with different expression levels of plat.