Redox Regulatory System in Semi-Sessile Amphibious Indian Apple Snail Pila Globosa for Future Ecotoxic Studies

Studies on oxidative stress on organisms especially on invertebrates are widely used as biomarkers in relation to the redox regulatory system to assess the freshwater environment. Despite broad availability of Indian apple snail Pila globosa , no data on its antioxidant and oxidative stress status are available. Therefore, determination of tissue-specific redox regulatory systems in P. globosa was the aim of this study. Snails were sampled from a non-polluted site by determination of oxidative stress parameters in hepatopancreas and foot muscle tissues. The levels of lipid peroxidation and total antioxidant capacity were found to be ten- and two fold higher, respectively, in hepatopancreas than foot muscle. Activity of different antioxidant enzymes was 20 to 100% higher in hepatopancreas than foot muscle. The concentration of small redox regulatory antioxidant such as GSH and AA was found to be 25 and 128% higher, respectively, in hepatopancreas. Thus, above tissue-specific antioxidant data can be used as base line data in P. globosa that may be used as biomarker species.