Analysis of the Composition of Different Instars of Tenebrio molitor Larvae using Near-Infrared Reflectance Spectroscopy for Prediction of Amino and Fatty Acid Content.

Insects are a sustainable protein source for food and feed. The yellow mealworm ( L.) is a promising candidate for industrial insect rearing and was the focus of this study. This research revealed the diversity of larvae in the varying larval instars in terms of the nutritional content. We hypothesized that water and protein are highest in the earlier instar, while fat content is very low but increases with larval development. Consequently, an earlier instar would be a good choice for harvest, since proteins and amino acids content decrease with larval development. Near-infrared reflectance spectroscopy (NIRS) was represented in this research as a tool for predicting the amino and fatty acid composition of mealworm larvae. Samples were scanned with a near-infrared spectrometer using wavelengths from 1100 to 2100 nm. The calibration for the prediction was developed with modified partial least squares (PLS) as the regression method. The coefficient for determining calibration (R) and prediction (R) were >0.82 and >0.86, with RPD values of >2.20 for 10 amino acids, resulting in a high prediction accuracy. The PLS models for glutamic acid, leucine, lysine and valine have to be improved. The prediction of six fatty acids was also possible with the coefficient of the determination of calibration (R) and prediction (R) > 0.77 and >0.66 with RPD values > 1.73. Only the prediction accuracy of palmitic acid was very weak, which was probably due to the narrow variation range. NIRS could help insect producers to analyze the nutritional composition of larvae fast and easily in order to improve the larval feeding and composition for industrial mass rearing.