Biocompatible Triple-Helical Recombinant Collagen Dressings for Accelerated Wound Healing in Microneedle-Injured and Photodamaged Skin

Skin rejuvenation procedures such as microneedling and laser resurfacing have gained global popularity in medical cosmetology, leading to acute skin wounds with persistent pain, erythema, and edema. A variety of dressings have been explored to repair these postoperative skin injuries; however, their inadequate biocompatibility and bioactivity may raise concerns about undesirable efficacy and complications. Herein, we developed biocompatible and nonirritating triple-helical recombinant collagen (THRC) dressings for accelerated healing of microneedle-injured and photodamaged acute skin wounds. Circular dichroism (CD) measurements of THRC from various batches exhibited triple-helical structure characteristics of collagen. Cell experiments using L929 fibroblasts revealed that THRC dressings possess superior biocompatibility and bioactivity, significantly elevating the proliferation and adhesion of fibroblasts. In vivo, skin irritation tests of New Zealand rabbits demonstrated that the THRC dressings are gentle, safe, and non-irritating. Histological analysis of the animal model studies in photodamaged skin wounds using H&E and Masson's trichrome staining revealed that 4 days of treatment with the THRC dressings effectively healed the damaged dermis by accelerating re-epithelialization and enhancing collagen deposition. In vivo studies of microneedle-injured rat defects showed that THRC dressings of varying concentrations exhibit the same rapid epithelialization rates at 48 h as commercial bovine collagen dressings. The highly biocompatible and bioactive recombinant collagen dressings may provide an advanced treatment of acute skin wounds, indicating attractive applications in postoperative care of facial rejuvenation.