Supplementary Methods, Tables 1 - 3; 5 - 6, Figures 1 - 10 from A DNA Methylation Prognostic Signature of Glioblastoma: Identification of NPTX2-PTEN-NF-κB Nexus

PDF file - 5025K, Supplementary Figure SF1: ROC analysis and risk stratification in three groups ; Supplementary Figure SF2: Risk score distribution of TCGA and our patients' cohort and and heatmap of 9 genes in TCGA cohort ; Supplementary Figure SF3: Kaplan meier analysis and Risk score distribution of Bent et al data set ; Supplementary Figure SF4: NFkb network and EMSA of NFkB protein ; Supplementary Figure SF5: Heat map of differentially expressed genes in low and high risk of REMBRANDT and Phillips et al datasets ; Supplementary Figure SF6: Kaplan meier analysis and landscape analysis of REMBRANDT and Phillips et al datasets ; Supplementary Figure SF7: NPTX2 expression level and methylation level correlation ; Supplementary Figure SF8: NPTX2 inhibits NFkB activity ; Supplementary Figure SF9: NPTX2 inhibits NFkB nuclear translocation ; Supplementary Figure SF10: NPTX2 inhibits NFkB activity by activating p53 dependent PTEN pathway.Table ST1: Kaplan Meier overall survival analysisin our patient set and the TCGA data set. ; Table ST2: Univariate and multivariate Cox regression analysis of methylation risk score with 9-gene predictor and 10-miRNA prognostic signature ; Table ST3: Multivariate Cox regression analysis of methylation risk score and other Prognostic markers using Bent et al. data set ; Table ST4: Differentially expressed genes between low and high risk ; Table ST5: Significantly enriched networks obtained from Metacore network analysis ; ST6: List of NFkB target genes overexpressed only in high-risk but not in Low risk compared to normal.