Significance of Bryophyllum pinnatum (Lam.) for green synthesis of anti-bacterial copper and selenium nanoparticles and their influence on soil microflora

Green copper nanoparticles (CuNPs) and selenium nanoparticles (SeNPs) are synthesised by combining the leaf extract of Bryophyllum pinnatum with 25 mM of copper sulphate (CuSO 4 ) and sodium selenite (Na 2 SeO 3 ), respectively. At 300 nm, the ultraviolet (UV) spectrum confirms the production of CuNPs and SeNPs. In CuNPs and SeNPs, the fourier-transform infrared (FTIR) spectrum reveals the presence of functional groups associated with bioactive compounds. X-ray spectroscopy shows that the properties of CuNPs and SeNPs are distinctive. Using energy-dispersive X-ray spectroscopy (EDX), copper and selenium with narrow, intense widths and great purity were identified. Depending on the species level, the minimum inhibitory concentrations (MICs) of CuNPs and SeNPs synthesised in a green approach ranged from 12 to 30 ppm. On soil microflora, synthesised NPs showed no detectable effect on microbial growth compared to the control. The present results demonstrate the benefits of a green approach to the generation of NPs with potent anti-bacterial activity and no negative impact on soil microbiota. The present findings illustrate the advantages of a green strategy for the development of NPs with potent antibacterial activity and no toxicity to soil microorganisms.