Analysis of the Therapeutics Effects of miR-126 on Proliferation, Migration and Invasion of Uterine Leiomyoma Cells

The purpose of this study was to investigate the effect of miR-126 on the proliferation, migration and invasion of uterine leiomyoma cells as well as on the growth of xenografts. The expression of miR-126 in uterine leiomyoma tissue and adjacent tissue was detected by qRT-PCR. The uterine leiomyoma cells were primarily isolated and cultured, and miR-NC and miR-126 mimics were respectively transfected into the uterine leiomyoma cells. The cell viability was detected by MTT assay. Cell migration and invasion were detected by Transwell cell assay. The expression levels of CyclinD1, MMP2 and MMP9 proteins were detected by Western blot. The miR-126 lentivirus vectors (LV-miR-126 and LV-NC) were constructed and transfected into uterine fibroid cells, respectively, to establish a mouse xenograft uterine fibroid model, and to detect the volume and infiltration rate of the xenografts. The positive rates of CyclinD1, MMP2 and MMP9 proteins in the xenografts were detected by immunohistochemistry. Compared with the adjacent tissues, the expression level of miR-126 in uterine leiomyoma tissues was decreased (p <0.05). Compared with the miR-NC group, the cell viability was decreased, the number of migrated and invaded cells was decreased and the protein levels of CyclinD1, MMP2 and MMP9 were decreased in the miR-126 group. Compared with the LV-NC group, the expression level of miR-126 in the LV-miR-126 group was increased (p < 0.05), the volume of the transplanted tumor was decreased, the infiltration rate was decreased and the positive rates of CyclinD1, MMP2 and MMP9 proteins were decreased. These finding suggest that the overexpression of miR-126 weakens the proliferation, migration and invasion of uterine fibroid cells, and inhibits the growth of xenografts uterine fibroid in mice.