Effects of long-term fertilization with contemporary Danish human urine, composted household waste and sewage sludge on soil nematode abundance and community structure

To bridge the organic-dependent barrier on nitrogen from low carbon/nitrogen (C/N) municipal wastewater, employing algal biochar supported nano zero-valent iron (ABC-nZVI) was investigated using A/A/O-MBR. Firstly, it can be seen that adequate carbon source is indispensable for the removal, since total nitrogen (TN) removal reached 77.89 % with the influent C/N of 7.8. Secondly, conducted in batch experiments with different doses of ABC-nZVI with/without active sludge, removal efficiency of total inorganic nitrogen (TIN) and the effective time achieved 84.94 % and 24 h with an ABC-nZVI dose of 300 mg/L, respectively. Thirdly, it was found that the duration of high -efficiency denitrification reached 9 h with the addition of 250 mg/L of ABC-nZVI to the anoxic tank of A/A/O-MBR, and the effluent ammonium nitrogen (NH4+-N) also meet the national discharge standard. Besides, biodiversity of both anoxic and aerobic sludge was apparently promoted with the addition of ABC-nZVI, while the lab-scale A/A/O-MBR could also be fully rehabilitated within 12 h. Finally, predicted through PICRUSt2, relevant abundance of func-tional genes involved in nitrogen metabolism could be enriched by nZVI addition. As an alternative supporting electron donor and mediator, ABC-nZVI can also be participated in the enhanced nitrogen removal in A/A/O-MBR at low C/N.