Lack of a protective effect of the Tmem106b “protective SNP” in the Grn knockout mouse model for frontotemporal lobar degeneration

Genetic variants in TMEM106B are a common risk factor for frontotemporal lobar degeneration and the most important modifier of disease risk in patients with progranulin ( GRN ) mutations (FTLD- GRN ). TMEM106B is encoding a lysosomal transmembrane protein of unknown molecular function. How it mediates its disease-modifying function remains enigmatic. Several TMEM106B single nucleotide polymorphisms (SNPs) are significantly associated with disease risk in FTLD-GRN carriers, of which all except one are within intronic sequences of TMEM106B . Of note, the non-coding SNPs are in high linkage disequilibrium with the coding SNP rs3173615 located in exon six of TMEM106B, resulting in a threonine to serine change at amino acid 185 in the minor allele, which is protective in FTLD- GRN carriers. To investigate the functional consequences of this variant in vivo, we generated and characterized a knockin mouse model harboring the Tmem106b T186S variant. We analyzed the effect of this protective variant on FTLD pathology by crossing Tmem106b T186S mice with Grn −/− knockout mice, a model for GRN -mediated FTLD. We did not observe the amelioration of any of the investigated Grn −/− knockout phenotypes, including transcriptomic changes, lipid alterations, or microgliosis in Tmem106b T186S/T186S × Grn −/− mice, indicating that the Tmem106b T186S variant is not protective in the Grn −/− knockout mouse model. These data suggest that effects of the associated SNPs not directly linked to the amino acid exchange in TMEM106B are critical for the modifying effect.