Hypoxic stress accelerates the propagation of pathological alpha-synuclein and degeneration of dopaminergic neurons

AimsThe etiology of Parkinson's disease (PD) is complex and the mechanism is unclear. It has become a top priority to find common factors that induce and affect PD pathology. We explored the key role of hypoxia in promoting the pathological propagation of alpha-synuclein (alpha-syn) and the progression of PD. MethodsWe performed PD modeling by conducting intracranial stereotaxic surgery in the unilateral striatum of mice. We then measured protein aggregation in vitro. The rotarod and pole tests were employed next to measure the damage of the phenotype. Pathological deposition and autophagy were also observed by immunofluorescence staining and protein levels measured by western blotting. ResultsWe demonstrated that short-term hypoxia activated phosphorylated (p)-alpha-syn in mice. We confirmed that p-alpha-syn was more readily formed aggregates than alpha-syn in vitro. Furthermore, we found that hypoxia promoted the activation and propagation of endogenous alpha-syn, contributing to the earlier degeneration of dopaminergic neurons in the substantia nigra and the deposition of p-alpha-syn in our animal model. Finally, autophagy inhibition contributed to the above pathologies. ConclusionHypoxia was shown to accelerate the pathological progression and damage phenotype in PD model mice. The results provided a promising research target for determining common interventions for PD in the future.