Doubled Haploid Rice Lines Production through Anther Culture of F-1 Derived from Abiotic Stress Tolerant Parents

Anther culture is one of the feasible technologies for accelerating rice breeding programs. This research aimed at determining the anther culture ability and doubled-haploid plants production of eight F(1)s derived from single crosses between indica rice parents that had a single or multiple abiotic stresses tolerance. The experiment was conducted using completely randomized design (CRD) with 15 replications. Medium for callus induction was based on N6 + 2.0 mg L-1 NAA +0.5 mg L-1 kinetin + 1 mM putrescine, while the regeneration medium was based on MS + 0.5 mg L-1 NAA + 2.0 mg L- kinetin 1 + 1 mM putrescine. Medium for rooting was MS + 0.5 mg L-1 IBA. The results indicated that all F1 populations gave varied responses to callus induction and plant regeneration. The F1 populations from HS4-11-1-2/B13926E-KA-23 had higher anther culture ability compared to other seven F-1 populations as indicated by the efficiency in callus formation and plant regeneration. Overall, the number of doubled haploid individuals (DHs) obtained was 158. F-1 populations using HS4-11-1-2 as female parent, HS4-11-1-2/B13926E-KA-23 and HS4-11-1-2/ CG8-93-1-1 contributed 45.4% and 34.9% doubled haploid individuals, respectively. The developed DHs lines need to be morphologically and agronomically characterized as well as screened under different combinations of abiotic stress to identify high-yielding and abiotic stress tolerant individuals for further rice breeding program.